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Cost effective and sustainable fermentation solutions - Estibio

Cost effective and sustainable

fermentation solutions

 

Pentocrobe® thermophilic microorganisms (463X and 463S) efficiently catalyse the anaerobic fermentation of sugars to ethanol for biofuels at high temperatures.

Estibio’s VHTP (very high temperature processing) fermentation platform harnesses the key metabolic pathways of Pentocrobe®, an in-house genetically modified microorganism, derived from the thermophilic species Thermoanaerobacter italicus, which enables simultaneous conversion of simple sugars as well as combined C5/C6 biomass sugars into bioethanol.

The Pentocrobe® thermophiles maximise productivity; producing near-pure ethanol. This is achieved by modifying the organism’s metabolic pathways by eliminating other side-products e.g. acetic acid and lactic acid through genetic engineering.

The combined thermophilic and anaerobic properties of the Pentocrobe® microorganisms enable continuous processing at elevated temperatures (~70° Celsius), resulting in less microbial contamination, and a continuous operation with lower cooling costs.

Pentocrobe® has been successfully tested on a range of lignocellulosic biomasses including wheat straw, sugarcane bagasse, corn cob and corn stover.

Estibio out-licenses its patented Pentocrobe® microorganisms. These can be used in a customer’s existing first generation (1G) biofuel plant, in 2G plants and/or can be combined with BioGasol’s Carbofrac® pretreatment system.

FEATURES

  • Ferments a wide range of sugars; glucose, xylose, arabinose, galactose, mannose and cellobiose simultaneously in continuous culture
  • Ferments xylo-oligomers and starch
  • Operates at temperatures between 60°C and 75°C
  • Tolerates high levels of acetic acid and high salinity
  • Tolerates high concentrations of 2-furaldehyde and other lignocellulosic biomass related inhibitors, when operated in continuous culture
  • Stable ethanol yield of 0.47-0.48 g/g of consumed sugar
  • High conversion (>95%) of glucose, xylose, arabinose, and cellobiose
  • pH range of 6.0-7.5
  • Ethanol productivity of 1-4 g/L/h
  • High ethanol titer

Bioethanol production

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